Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture.
نویسندگان
چکیده
1. A simple axenic medium suitable for the growth of the myxamoebae of a strain of the cellular slime mould Dictyostelium discoideum is described. 2. Procedures suitable for the growth of this strain in liquid and on solid media are described. 3. Conditions suitable for initiating the cell differentiation of myxamoebae grown axenically are described.
منابع مشابه
Metabolism of the cellular slime mould Dictyostelium discoideum grown in axenic culture.
1. The DNA, RNA, protein and carbohydrate contents of myxamoebae of Dictyostelium discoideum strain Ax-2 were measured after growth on bacteria or in various axenic media. 2. Myxamoebae grown in the different axenic media have similar DNA, RNA and protein contents, but there are marked differences in the contents of glycogen and free sugars. The DNA and protein contents of myxamoebae grown on b...
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1. The specific activities of fl-N-acetylglucosaminidase, acid phosphatase, ac-mannosidase, P-glucosidase, UDP-glucose pyrophosphorylase and alkaline phosphatase have been determined in myxamoebae of the cellular slime mould Dictyostelium discoideum Ax-2 grown on different media and in different phases of the growth cycle. 2. Variations in enzymic composition occur with changes in growth medium...
متن کاملControl of cell differentiation in the cellular slime mould Dictyostelium discoideum.
s of Communications 565th Meeting of the Biochemical Society University of Stirling 9 and 10 September 1976 CONTROL M ECH AN ISMS I N D I FFER ENTl AT1 ON AND DEVELOPMENT: a Colloquium organized by L. Stevens (Stirling) Control of Cell Differentiation in the Cellular Slime Mould Dictyostelium discoideum
متن کاملA defined minimal medium for axenic strains of Dictyostelium discoideum.
A defined, minimal medium for the cellular slime mold Dictyostelium discoideum is reported.
متن کاملA neutral ceramidase homologue from Dictyostelium discoideum exhibits an acidic pH optimum.
The nucleotide sequence reported for the Dictyostelium discoideum ceramidase is available on the DNA Data Bank of Japan (DDBJ). Ceramidases (CDases) are currently classified into three categories (acid, neutral and alkaline) based on their optimal pHs and primary structures. Here, we report the first exception to this rule. We cloned the CDase cDNA, consisting of 2142 nucleotides encoding 714 a...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 119 2 شماره
صفحات -
تاریخ انتشار 1970